A rapid method for detection of pentachlorophenol(PCP) residue was established based on gold immunochromatography assay.The colloidal gold strip for PCP was developed successfully.Colloidal gold particles with a size of 20 nm were prepared by trisodium citrate disoxidation method.PCP and anti-mouse goat immunoglobulins were jet-positioned onto a nitrocellulose membrane.Nitrocellulose membrane,gold jet pad,sample pad and absorbent paper were assembled and cut into detecting card,respectively.Through the strip color shades,the detection of PCP residue samples was performed.The detection limit of the colloidal gold strip was 10 ng/mL for PCP in 5 min,and that all needed reagents were included in the strip.This method is characteristic of simplicity,high sensitivity and low cost to achieve the rapid detection of PCP residue.