In this paper,a new method based on smartphone was proposed for the rapid detection of organophosphorus residues pesticide,and effects on the detection results with three smartphones of different bands were also studied.In order to minimize the influences of ambient light on the images,a surface source based on light-emitting diode(LED) was used,and the accuracy of the detection was also improved.In the experiment,the LED surface source was placed upon the samples vertically and the smartphone was arranged in front of the samples to capture the images.The results are consistent with that obtained by the UV-Vis spectrophotometer.