An electrode-modified by gold nanoparticles(AuNPs) and polymerized film of melamine(PMel)(PMel/Au/GCE) was fabricated.Electrochemical impedance spectroscopy(EIS) was used for analyzing the modified electrode.The electrochemical behavior of rutin was investigated by cyclic voltammetry(CV)，and a couple of sensitive and reversible redox peaks were obtained with an oxidation peak at 0.422 V and a reduction peak at 0.374 V.It was found that the oxidation peak current(Ipa) and the reductive peak(Ipc) current at PMel/Au/GCE were obviously larger than those at bare glassy carbon electrode(GCE) and the electrode modified by polymerized film of melamine(PMel/GCE)，which indicated that the sensitivity for detection of rutin was improved.Experimental parameters，including pH value of buffer solution，electro-deposited time of AuNPs，electro polymerized time of PMel and scan rate were optimized.The differential pulse voltammetry(DPV) was used for the quantitative analysis on rutin.Good linear relationships between current intensities and rutin concentrations were obtained in the ranges of 7.8×10-9 -1.2×10-6 mol/L and 1.2×10-6-1.5×10-5 mol/L，with correlation coefficients(r2) of 0.997 and 0.993，respectively.The detection limit(S/N=3) was 5.5×10-9 mol/L.The modified electrode was applied in the determination of rutin in rutin tablets with recoveries of 96.4%-101.8%.