Bleomycins（BLMs）,a family of glycopeptides-derived antibiotics,are widely used as available chemotherapeutic agents against a variety of cancers.In the presence of Fe（Ⅱ） ions,BLM form a binary BLM?Fe（Ⅱ）complex that can activate molecular oxygen.Herein,it is demonstrate for the first time that BLM?Fe（Ⅱ） complex may possess an intrinsic peroxidase-like activity and could catalyze 2,2’-azino-bis（3-ethylbenzthiazoline-6-sulfonic acid）（ABTS） into colored product in the presence of hydrogen peroxide.Experimental results revealed that the catalytic activity of BLM?Fe（Ⅱ） complex is strongly dependent on pH value and temperature,which is similar to natural enzymes such as horseradish peroxidase.The conditions for maximum catalytic efficiency were determined to be pH 6.0 and 30 ℃.Taking advantage of the color reaction of ABTS catalyzed by BLM?Fe（Ⅱ） complex,a simple and rapid colorimetric method for the detection of BLM was thus established.Under the optimal conditions,a good linear relationship was obtained over BLM concentration in the range of 0.1-2.5 μmol/L with a detection limit of 14.1 nmol/L.With the advantages of good reproducibility and high selectivity,the method is anticipated to have a practical potential for BLM analysis in clinical applications.