A MWCNTs loading cobalt-iron mixed oxide modified electrode was fabricated by using cyclic voltammetry.The electrocatalytic activity of the modified electrode for the oxidation of glucose was investigated by cyclic voltammetry and amperometric method．The results showed that the modified electrode exhibited a high electrocatalytic activity for the oxidation of glucose.Based on this,an amperometric method for the determination of glucose was established.Under the optimum conditions,the oxidation peak current had linear relationships with concentrations of glucose over the ranges of 2.0×10-7-4.2×10-5 mol?L-1 and 4.2×10-5-4.9×10-4 mol?L-1,with sensitivities of 242.7 μA?（mmol?L-1）-1 and 114.8 μA?（mmol?L-1）-1,respectively.The detection limit（3sb） was 5.0×10-8 mol?L-1.The proposed method was successfully applied in the detection of glucose in serum.