An ultra high performance liquid chromatography-tandem mass spectrometric（UHPLC-MS/MS） method was established for the determination of 10 sedative（azaperol,azaperone,xylazine,haloperidol,promethazine,acepromazine,propionylpromazine,chloropromazine,methaqualone and diazepam） residues in pork,beef and mutton.After enzymolysis,10 veterinary drugs were extracted with acetonitrile and cleaned up with an Oasis MCX SPE column.The analysis of the sedatives were achieved on an ACQUITY UPLC CSH C18 column and detected by electrospray ionization-tandem mass spectrometry in the multiple reaction monitoring（MRM） mode.The calibration curves of ten sedatives showed good linearities（r2≥0.993 6） in the concentration range of 0.5-100 μg/L.The limits of detection（LODs,S/N≥3） were not more than 0.5 μg/kg，and the limits of quantitation（LOQs,S/N≥10） were not more than 1.0 μg/kg.The average recoveries of ten sedatives ranged from 82.0% to 116.0% with relative standard deviations（RSDs） of 0.6%-12.8% at the spiked levels of 1.0,5.0,50.0 μg/kg.The results showed that the method was sensitive,and accurate,and was suitable for the determination of sedative residues in animal originated food．