A molecular imprinted polymer of cross linking chitosan was developed with Cr3+ as template molecule and glutaraldehyde as crosslinking agent.The adsorption properties of the molecular imprinted polymer were studied and the result showed that the imprinting crosslinked chitosan resin has good capacities of both absorption and selection for Cr3+.The imprinting crosslinked chitosan resin was used to prepare a solid phase extraction column,which was connected to a chemiluminescence analysis system as a CL cell.By using the catalytic action of Cr3+ on the chemiluminescence of luminol-H2O2 system,a new flow injection combined with on-line preconcentration and separation chemiluminescence method was set up for the determination of Cr3+.The linear range for the proposed method was in the range of 1.0×10-8-1.0×10-5 g/L.The detection limit was 3.7×10-9 g/L，and the relative standard deviation was 3.8%（ρCr3+=1.0×10-6 g/L,n=11）.After running more than 180 times,the separation efficiency of the solid phase extraction column did not change obviously.