A rapid,sensitive and accurate method for the simultaneous determination of 5 kinds of chlorophenols(CPs) in seawater samples by dispersive magnetic solid phase extraction-liquid chromatography(dMSPE-HPLC) was developed.The seawater samples were firstly enriched with pentachlorophenol-imprinted amino-functionalized nano-Fe3O4-polymer magnetic composite material(nFe3O4@MIPNH2-polymer),then separated on a C8 analytic column(250 mm×4.6 mm×5.0 μm) using methanol-NH4Ac solution(70∶30) as mobile phase and detected at 230 nm via UV detector.The results showed that under the optimal condition,the calibration curves were linear in the range of 1-5000 ng/L with correlation coefficients(r2) higher than 0.9989 for target chlorophenols.The spiked recoveries were between 86.5% and 98.8% with relative standard deviations(RSDs) of 0.8%-8.6%.The limits of detection(LODs) were in the range of 0.18-1.20 ng/L and the limits of quantitation(LOQs) were between 0.6 ng/L and 4.0 ng/L.The developed method could be applied in the routine analysis of chlorophenols in seawater samples.