A rapid and sensitive analytical method for the determination of cuppric nonyl phenolsulfonate residues in tobacco and soil samples was developed and validated based on QuEChERS（Quick,Easy,Cheap,Effective,Rugged and Safe） and high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）.Factors affecting the decomplexation efficiency by different compounds were investigated.The effects of type and volume of dispersive solvent on the clean up of tobacco and soil extracts were also assessed.Residues in the samples were decomplexated by sodium sulfide,extracted with acetonitrile and then cleaned up with primary secondary amine（PSA）.The product nonyl phenol sulfonic acid in the extract was separated by gradient elution using a mobile phase of acetonitrile-water and then analyzed by electrospray ionization in positive ion mode（ESI+） under multiple reaction monitoring（MRM） mode.Linearity was assessed by calibration curve in blank matrix to compensate for matrix effect.Good linearities（r >0.99） were obtained for nonyl phenol sulfonic acid in the matrix matched standards in the range of 0.001-1.0 mg?L-1.The average recoveries at spiked levels of 0.01-2.0 mg?kg-1 were in the range of 84.7%-92.5% for green tobacco leaf,87.1%-103.2% for dried tobacco leaf and 83.0%-90.9% for soil,with relative standard deviations（RSDs） of 6.9%-8.3%,5.8%-10.6% and 6.0%-9.0%,respectively.The limits of detection（LOD） were 5.5×10-5 mg?kg-1,while the limits of quantitation（LOQ） were determined as 0.01 mg?kg-1 for green,dried tobacco leaf and soil.This method is easy,quick and effective,and could be applied in the determination of cupric nonyl phenolsulfonate in tobacco and soil samples.