A gas chromatography-mass spectrometric method was developed for the simultaneous determination of three prohibited bactericides（chlorothalonil，myclobutanil and captafol） in anti-acne cosmetics.Lotion-type cosmetic samples were injected for analysis after extracted with methanol and dried with anhydrous sodium sulfate.Grease-type cosmetic samples such as emulsion and cream were first demulsificated with sodium chloride，then extracted with methanol and dried using anhydrous sodium sulfate.As a following clean up step，dispersive solid-phase extraction（DSPE） was utilized to minimize the interference of co-extracted substances.The chromatographic separation was performed on an HP-5MS（30 m×0.25 mm×0.25 μm） capillary column.The mass spectrometric analysis was conducted with electron ionization in selected ion monitoring（SIM） mode.The characteristic fragment ions were used for qualification,and the external standard method was employed for quantitation.Under the optimized conditions，this method showed a good linearity（r2≥0.997） in the appropriate concentration ranges.The limits of detection（LODs） for chlorothalonil，myclobutanil and captafol were 0.1，0.2,0.01 mg/kg，and the limits of quantitation（LOQs） were 0.2，0.5,0.02 mg/kg，respectively.The recoveries of three analytes ranged from 80.5% to 111.3%，with relative standard deviations（RSDs，n=6） of 1.8%-10.3%.The proposed method was validated with accuracy，reliability，simplicity and sensitivity，and could provide a scientific basis and technical support for the inspection and quality control of anti-acne cosmetics.