Microfluidic technologies,arising from multi disciplinary intersection,has become an indispensable platform in natural science because of its characteristics of miniaturization,integration and high throughput.In this review,the recent applications of microfluidic technologies in life science research were outlined.The applications were divided into four categories according to the scale of the analyzed objects:biomacromolecule analysis,cell based analysis,organs on chips,and studies on small model organisms.And the development prospects of this microfluidic technologies were proposed.