As a low consumed,rapid,efficient technology,microchip electrophoresis which is also portable,integrated,and high-throughput,plays an important role in application of clinical analysis.In this paper,the advantages of microchip electrophoresis are introduced concisely.The latest advances of microchip electrophoresis in clinical applications are reviewed,including the developments in small molecules,amino acids,proteins,nucleic acids,as well as cells.And a newly developed method will be used in two-dimensional analysis,and a new wide channel microchip（WCC） system is established.The developments for the future are also discussed.