A novel method was proposed for the determination of oxytetracycline by capillary electrophoresis（CE） with electrochemiluminescent（ECL） detection based on the enhancement effect of ECL intensity in the tris（2,2’-bipyridine） ruthenium（Ⅱ）（Ru（bpy）2+3-ECL） system by oxytetracycline.The sensitivity was further improved by 3.5 fold by the field amplified sample injection technique.The best result was achieved with a sampling voltage of 10 kV and an injection time of 6 s in 8 mmol?L-1 borate buffer（pH 8.0） at a detection voltage of 1.22 V.The consumed time for one run was about 9 min.A linear range of 20-200 μg?L-1（r=0.995 3） with a limit of detection（S/N=3） of 5 μg?L-1 was obtained by the proposed method.The intra-day relative standard deviations（RSDs） of relative ECL intensity and migration time for five replicated trials of 100 μg?L-1 oxytetracycline were 2.4% and 2.1%,respectively,and the inter-day RSDs of relative ECL intensity and migration time for five replicated trials of 100 μg?L-1 oxytetracycline were 3.6% and 2.8%,respectively.The developed method was successfully applied in the detection of oxytetracycline residue in a honey sample.The average recoveries were in the range of 91.5%-105.2% at spiked levels of 200,1 000,2 000 μg?kg-1.The limit of detection for the proposed method was 10 μg?kg-1 in honey matrix,which was lower than the maximum residue limit of oxytetracycline in food.The results indicated that the proposed method had an application potential in food analysis.