Drug screening is to apply the appropriate screening methods and screening technologies to extract the pharmacological active compounds from vast amounts of compounds.Drug screening is the key to improve the drug research efficiency,accelerate research cycle,cut down research cost and reduce investment risk,which makes the new drug research and development can be sustained.Drug screening new technologies have also become the important content of pharmaceutical research.More and more drug screening technologies have been developed,such as high throughput screening technology,high content screening technology,surface plasmon resonance technology and microfluidic chip technology,etc.These new drug screening technologies and their application are reviewed in this article.