Under alkaline condition, gold nanoparticles(AuNPs) could enhance the chemiluminesence(CL) of luminol-silver nitrate system,and amitriptyline hydrochloride could greatly enhance CL signals. Based on this,a new simple CL method was developed to detect the content of amitriptyline hydrochloride.The experimental conditions were optimized,and the possible mechanism of the system was discussed.Under the optimal conditions,the relative CL intensity was linearly proportional to amitriptyline hydrochloride concentration in the range of 3.0×10-9-3.0×10-7 g/mL,with a correlation coefficient of 0.999 4 and a detection limit(S/N=3) of 2.1×10-9 g/mL.The RSD (n=11) was 2.0% for 5.0×10-8 g/mL amitriptyline hydrochloride.The method was applied in the determination of amitriptyline hydrochloride tablets,and the result was in consistent with the standard method.