1H NMR spectroscopy combined with multivariate statistical method was introduced in this paper to explore the regulation effect of Gandouling on basal ganglia injury in copper-laden rats.24 male Wistar rats were randomly divided into control group,model group and Gandouling group,with 8 rats in each group.The rat model was established by copper-laden method.From the 7th week of modeling,Gandouling group was given Gandouling formula via gavage.Compared with the control group,the index of apoptosis cells in basal ganglia of model group increased significantly(p<0.01),and glutamine,uridine and threonine in basal ganglia were increased(p<0.05) while mannitol,adenosine,N-acetylaspartate,glutamate, N-acetylglutamine asparagine, acetate, aspartate,myo-inositol and ATP were decreased(p<0.05).Compared with the model group,the index of apoptosis cells in basal ganglia of Gandouling group was significantly decreased(p<0.01),and glutamine,uridine,acetate and aspartate were reduced(p<0.05) while mannitol,adenosine,threonine,N-acetylaspartate,glutamate, N-acetylglutamine, asparagine,myo-inositol and ATP were elevated(p<0.05).The results showed that Gandouling could influence the metabolism of basal ganglia in copper-laden rats,which has a certain therapeutic effect on damage caused by copper.The mechanism may be restoration of the functions of neurons and glial cells,through adjusting the detoxification of ammonia and metabolism of excitatory amino acid neurotransmitter and interfering the energy metabolism.