A method was developed for the simultaneous determination of hexoestrolum,diethylstilbestrol,dienestrol,estrone,17β-estradiol and estriol in feed by solid phase extraction and gas chromatography-mass spectrometry(GC-MS).After extracted with ethyl ether and cleaned up on an HLB phase extraction column,six kinds of estrogens were derived and quantified by GC-MS.The external standard calibration curves were used for the quantification.The results showed that the high linear ranges from 2.5 ng/mL to 250 ng/mL for hexoestrolum and from 5 ng/mL to 500 ng/mL for five other estrogens were obtained with correlation coefficients (r2) not less than 0.99.The limits of detection were in the range of 3-8 μg/kg and the average recoveries were 75.9%-96.3%.The results demonstrated that the developed method could be applied in the qualitative and quantitative determination of six kinds of estrogens in feed with low limit of detection and high accuracy.