A molecularly imprinted solid phase extraction coupled with high performance liquid chromatographic (HPLC) method was firstly developed for the determination of clenbuterol hydrochloride residues in pork liver.The chromatographic conditions were as follows:the column:ASB C18 column(250 mm × 4.60 mm,5 μm),mobile phase:methanol-water (45∶55,pH 3.0),UV detection wavelength:243 nm,sample volume:10 μL,flow rate:0.6 mL/min,column temperature:30 ℃.Molecularly imprinted solid phase extraction conditions were as follows:solvent of sample loading:double distilled water,flow rate of loading:0.6 mL/min;elution solvent:methanol solution containing 1%trifluoroacetic acid.Under the optimal conditions,the calibration curve was linear in the range of 0.432-4.32 μg/mL,with linear equation of y=30 358x-307.74(r=0.999 0).The spiked recoveries were in the range of 84.7%-92.0%,with RSDs of 1.5%-1.8%.The accurancy and precision of the method could satisfy the requiremennts for residuals analysis.