Carbohydrate antigens CA125（Ag） are the most important markers in early stage of tumor and cancer stage.Antibodies Ab125（Ab） to carbohydrate antigens were labeled to form probe-labeled antibodies LP-Ab using the prepared two-photon fluorescence probe LP.On the basis of the antigen-antibody specificity affinity principle，LP-Ab could combine with carbohydrate antigen into a complex LP-Ab?Ag which emitted strong two-photon fluorescence to accomplish the high sensitive biopsy and the real-time dynamic high-resolution imaging of the tumor markers.The easy，simple and quick method could not only overcome photodamage，photobleaching and tissue auto-fluorescence disturbance，but also be used to observe and study the dynamic transfer mechanisms of the tumor markers in cells and the mechanism of carcinogenesis.The research in this project is very useful in the early diagnosis and prevention of tumor and canceration.