New molecularly imprinted polymers(MIPs)for recognizing four tropane alkaloids(anisodine,scopolamine,anisodamine,atropine) were synthesized by precipitation polymerization using anisodine(ASD)as template,methacrylic(MAA)as functional monomer,trimethylolpropane trimethacrylate(TRIM)as crosslinker and acetonitrile as porogen.The optimal functional monomer and the ratio were selected by UV spectrum method.Comparative determination of the prepared MIP and NIP were carried out by equilibrium adsorption experiment.The results showed that the MIPs possessed the analogous-specific adsorptivity toward four TAs.Scatchard anilysis displayed that the apparent maximum binding capacities(Qmax) are 7.53,10.90,24.27,11.04μg/mg, respectively, and relative selectivity coefficients(IF) are 3.58，1.49，1.62，2.25,respectively.By using the MIPs as sorbent in solid-phase extraction column,a molecularly imprinted solid phase extraction combined with high performance liquid chromatographic(MISPE-HPLC) method was developed for the high performance enrichment and fast separations of four TAs in Przewalskia tangutica Maxim..Good linear calibration curves were obtained in the concentration range of 2-250μg/mL(r2≥0.9999) with LODs of 0.26-0.39μg/mL.The method gave excellent recoveries(70.0%-96.3%) and precision(RSDs≤5.7%,n=3) at 10,50,100μg/g spiked levels.The results indicated that the molecularly imprinted solid-phase extraction(MISPE)with slight matrix interference,highly repeated usage and expectant separation effect,greatly improved extraction efficiencies for four TAs in Przewalskia tangutica Maxim..