A novel poly neutral red film modified carbon paste electrode(PNR/CPE) was prepared by chemical initiation-electrochemical polymerization.The electrochemical properties of the modified electrode were characterized by cyclic voltammetry(CV) and electrochemical impedance spectroscopy(EIS),the surface morphology of the ploy neutral red film was observed by scanning electron microscopy (SEM).Meanwhile,the membrane structure of PNR was tested and analyzed by ultraviolet visible absorption(UV-Vis) and infrared absorption spectrometry(IR).The result showed that the neutral red was modified successfully on the surface of carbon paste electrode,and the surface of PNR/CPE had a specific three dimensional structure,in which the electroccatalytic performance was improved with the increase of the electroactive sites of the surface.Under the optimal experimental conditions,the modified electrode was applied in the detection of herring sperm DNA(hsDNA).The result indicated that a pair of obvious redox peak appeared on the PNR/CPE,the peak currents and concentrations of hsDNA had a good linear relationship in the range of 1.0×10-6-8.0×10-5 mol/L with a detection limit of 1.0×10-7 mol/L.