Based on the enhancement of ECL intensity of Ｒu(bpy)2+3 in the presence of diuron，a new ECL method was established for the determination of diuron at the glassy carbon electrode modified with Nafion film of 3D graphene and SiO2@Ru.The 3D graphene which has the ability to enhance the intensity of electrochemiluminescence(ECL) was synthesized by a one-pot hydrothermal method.In addition，Ｒu(bpy)2+3-doped silica nanoparticle was prepared by water-in-oil microemulsion method to increase the ECL intensity.Under the optimum conditions，the ECL intensity was well proportional to the logarithmic of diuron concentration in the range of 9.08×10-11-9.08×10-7 mol/L with a detection limit as low as 1.18×10-12 mol/L.The RSD for 2.00×10-8 mol/L diuron(n=10) was 4.2%,which indicated that this method had a good repeatability.The method was applied in the determination of diuron in vegetable samples with recoveries of 99.3%-110.8%.