A new method using TurboFlow online cleanup combined with tandem mass spectrometry was developed for the rapid and simultaneous determination of six polyether antibiotics residues,e.g.lasalocid,salinomicin,monensin,narasin,maduramycin and nigericin in animal-originated foods of animal origin.The milk，chicken and egg samples were extracted with acetonitrile，and the extracts were purified on a Cyclone-p column.After being eluted from the clean-up column，the analytes were separated on a Thermo Scientific Hypersil-Gold C8(150 mm×2.1 mm，5 μm) column and detected by electrospray mass spectrometry in positive ionization mode with multiple reaction monitoring.Under the optimized conditions，the linear ranges of six polyether antibiotics were in the range of 1-100 μg/L with correlation coefficients higher than 0.999.The limits of quantitation(LOQ) for all samples were 1 μg/kg.The recoveries from milk，chicken and egg samples were in the range of 71.9%-109.0% with relative standard deviations of 1.5%-14.9%.