A method for the simultaneous determination of 16 polycyclic aromatic hydrocarbons(PAHs) in tobacco by four-channel chromatograph purification coupled with gas chromatography-mass spectrometry(GC-MS) was developed.The tobacco samples were extracted by the method of soxhlet extraction with dichloromethane,and the extract was cleaned with four-channel purification chromatograph.The eluent were determined by GC-MS in SIM mode,and quantitated by a method of external standard.The procedure of separation and purification for fresh tobacco extraction was optimized.The results indicated the calibration curves of 16 PAHs had good linear relationships in the range of 10-1 000 μg/kg(r＞0.99).The detection of limits(LODs) and method detection limits(MDLs) were 0.35-1.75 μg/kg and 1.01-2.87 μg/kg,respectively.The recoveries at three spiked concentration levels were in the range of 81.0%-107.8%with relative standard deviations(RSD,n=6) of 2.7%-8.0%.The method is suitable for the determination of PAHs in tobacco or other crops with easy operation,rapidness,high repeatability and strong practicability.