A method was developed for the determination of ellagic acid(free ellagic acid and total ellagic acid) in strawberry by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).In this work，the free ellagic acid was extracted from strawberry with 0.1%trifloroacetric acid-methane，purified by C18 dispersive solid phase extraction，and determined by UPLC-MS/MS.Total ellagic acid was hydrolyzed to free ellagic acid with 2 mol/L trifloroacetric acid aqueous，then purified with C18 dispersive solid-phase extraction column，and determined by UPLC-MS/MS.Sample solution was separated on a C18 column by gradient elution using methanol-0.5%formic acid aqueous as mobile phase.The linear range of 10-500 ng/mL for ellagic acid was obtained，with correlation coefficient of 0.998 1.The limit of quantitation was 0.5 mg/kg for free ellagic acid and 5.0 mg/kg for total ellagic acid.The recoveries at low，medium and high spiked levels ranged from 867%to 1136%with relative standard deviations(RSD) not more than 10%.The results indicated that this approach was simple，sensitive and accurate，and was applicable for the determination of content of ellagic acid in strawberry.