An original method for the simultaneous determination of bisdemethoxycurcumin，demethoxycurcumin and curcumin in plasma，muscle，liver and kidney of yellow catfish（Pelteobagrus fulvidraco），bluntnose black bream（Megalobrama amblycephala） and grass carp（Ctenopharyngodon idellus） was established by ultra high performance liquid chromatography with ultraviolet detection（UPLC-UV）.The sample was extracted using acetonitrile containing 0.01% acetic acid，and the moisture of sample was removed with anhydrous sodium sulfate，then n-hexane was used for removing fat in sample.After that，the sample prepared was separated on an ACQUIT UPLC BEH C18 column and determined at 428 nm.Good linearities were observed in the range of 0.01-5.00 mg/L，and the correlation coefficients for bisdemethoxycurcumin，demethoxycurcumin and curcumin were 0.998 7，0.999 8 and 0.999 6，respectively.The average recoveries of 3 analytes in fish tissues at the spiked level of 0.025-1.00 mg/kg ranged from 64.7% to 102.2%,with relative standard deviations（RSDs） of 0.69%-10.8%.The detection limits（LODs） and quantitation limits（LOQs） for 3 analytes in fish tissues were all 0.010 mg/kg and 0.025 mg/kg，respectively.The presented method was firstly applied in the investigation on the pharmacokinetic profile of curcumin in bluntnose black bream.