A multistage liquid-liquid microextraction(LLME) coupled with GC-MS method was developed to determine squalene in Chinese liquor.After diluting the liquor sample,a multistage microextraction was carried out using pentane-diethyl ether mixture(1∶3,by volume),then the organic phase was concentrated with nitrogen,and analyzed by GC-MS.The result indicated that squalene had a good linearity in the range of 0.62-159.88 μg/L,with a correlation coefficient of 0.995 5.The detection limit of squalene was as low as 0.17 μg/L.The average recoveries of squalene at spiked levels of 2,20,40 μg/L were 78.1%,85.0%,103.5%,respectively,with relative deviations of 12.1%,8.9%,7.0%,respectively.With the advantages of simplicity,accuracy,high-extraction efficiency and low consumption of organic solvent,this method was suitable for the quantitative analysis of squalene in Chinese liquor.