In this study,concentrated sulphuric acid was employed for the determination of hydrocortisone biotransformation rate,which could be monitored via dual wavelength analysis.A rapid and simple method for the determination of hydrocortisone biotransformation rate was developed by combining the well-known Lambert Beer law and the 3D data modeling method.The method recoveries were in the range of 96.9%-110.8%.The validation results,which were consistent with that of HPLC method,showed that the method could meet the needs for preliminary screening of Absidia coerulea via high-throughput system.This study has a good application prospect in breeding Absidia coerulea of high specific biotransformation ability.