The effect of tobacco with herbal additives on the global metabolome of rat lung was studied using liquid chromatography-mass spectrometry(LC-MS).Lung samples from control rats，normal tobacco smoked rats and a certain commercial tobacco(a specific tobacco with herbal additives)smoked rats were analyzed，and the groups were exposed to smoke for one week，half month and one month，respectively.The data were processed by the method of partial least squares discriminant analysis(PLS-DA).The potential biomarkers were screened out according to VIP(Variable Importance in the Projection) and identified by database and standards.The PLS-DA results of different tobacco exposed periods showed that the metabolism of both the normal tobacco exposed group and the certain commercial tobacco exposed group were affected，mainly including phospholipid metabolism and fatty acid metabolism. In particular，two lung samples from rats exposed to normal tobacco were abnormal significantly as a result of suffering from a serious oxidative damage. Overall，especially prolonged exposure to smoke，the smoke damage of the certain commercial tobacco smoked rats was lower than that of the normal tobacco smoked rats，indicating that adding some herbal additives to tobacco could contribute at least partly to reduce the harm caused by smoking.