A novel and simple mass spectrometric method based on microwave plasma torch(MPT) was developed for the rapid detection of aflatoxin B1(AFB1) in rice without sample pretreatment.The MPT mass spectra of AFB1 were obtained,and the quantitative analysis mainly was based on the characteristic fragments of AFB1(m/z 285).The results showed that there was a good linear relationship in the concentration range of 0.5-20 μg/L with correlation coefficient(r2) of 0.999 4,and the evaluated limit of detection(LOD) was 0.12 μg/kg.The direct detection of AFB1 in some kinds of rice were conducted,with recoveries of 95.8%-107.2% and relative standard deviations(RSD,n=9) of 2.2%-10.1%With the help of principle component analysis(PCA),the moldy rice and normal rice could be rapidly distinguishedWith the advantages of rapidness and high sensitivity,the MPT-MS is a powerful tool for the high-throughput detection of moldy rice,and also shows a great potential in the monitoring of cereal and oil,animal,plant food,etc.