A method based on ultra high performance liquid chromatography-high resolution mass spectrometry was developed to separate benzoic acid，sorbic acid，acesulfame-K and saccharin sodium in honey.The targeted compounds in honey samples were extracted with methanol-water mixtures（10∶90）,and separated on a Diamonsil Plus C18 column with 0.5 mmol/L ammonium acetate-methanol as mobile phase by gradient elution.The qualitative and quantitative analyses were operated under t-MS2 by high resolution mass spectrometry with the external standard method.The results indicated that four analytes had good linearities in the concentration range of 0.02-2.0 mg/L with correlation coefficients of 0.998 7-0.999 7.The quantilition limits for benzoic acid，sorbic acid,acesulfame-K and saccharin sodium were 0.5，0.5，0.3,0.3 mg/kg，respectively.The recoveries of four compounds at three spiked levels were in the range of 89.0%-104.0%，with relative standard deviations（RSDs） of 2.0%-7.9%.The method was simple and rapid，and was suitable for the determination of benzoic acid，sorbic acid and acesulfame-K，saccharin sodium in honey.