A fluorescence resonance energy transfer（FRET）system between fluorescence carbon dots（CDs,donor）and fluorescein（FAM,acceptor）was constructed,and a novel method for the sensitive and selective determination of aspirin（ASP）was accordingly proposed.It was found that in pH 7.0 Tris-HCl buffer，effective energy transfer from CDs to FAM occurred,which resulted in a great enhancement of the fluorescence intensity of FAM.Upon the addition of ASP,fluorescence quenching of FAM occurred,and the change of FAM fluorescence intensity was in a good linearity（r=0.999 6）with ASP in the concentration range of 1.0-150.0 μg/mL.A novel fluorescence quenching platform for the determination of ASP was constructed.Under the optimum conditions,the detection limit of ASP for this method was 0.33 μg/mL（3δ/k,n=11）,and the spiked recoveries ranged from 97.1%to 105.3%with RSD（n=6）not more than 4.6%.The result showed that common relevant substance,cations and anions did not interfere with the detection of ASP.