A method was explored for the preparation of affinity chromatographic support， based on the hydrophilic gigaporous polymer microspheres using Protein A as affinity ligands.The effect of H5IO6 on amount of Protein A was evaluated.The morphology of the microspheres was observed by scanning electronic microscopy.The result showed that the throughput pores were maintained in the affinity media.At different flow rates(361-3 600 cm/h)，the dynamic capacity of human Ig G on the affinity supports were determined,and the result indicated the capacity at 3 600 cm/h decreased about 10% in comparison with that at 361 cm/h.The above results indicated that this affinity support could meet the requirement for rapid and high-throughput separation of proteins.