A method was established for the determination of six arsenic species including arsenite(As(Ⅲ)),arsenate(As(V)),monomethylarsine (MMA),dimethyarsine (DMA),arsenobetaine(AsB) and arsenocholine(AsC),in animal derived traditional Chinese medicines by liquid chromatography coupled with inductively coupled plasma mass spectrometry(LC-ICP-MS) assisted by microwave acid extration.After microwave extraction with 1% HNO3 solution within 10 min and centrifugation,the sample was purified with solid phase extraction SPE C18 and 0.45 μm hybrid filter membrane,and then eluted with 25 mmol/L NH4H2PO4 (pH 6.7) –ethanol(99∶1,by volume) as mobile phase by isocratic elution.The arsenic species could reach a baseline separation within 10 min.The results showed that the linear ranges for six arsenic species were in the range of 1.0-100.0 μg/L with correlative coefficients of 0.999 5-0.999 7.The limits of detection (LOD) for six anions were in the range of 0.24-1.0 μg/kg with relative standard derivations(RSD) of 0.96%-2.0%.The method was used in the determination of arsenic speciation in six samples involved pberetima,leech,cuttlebon,ootheca mantidis,shell of abalone and ventriculi galli mucosa.The spiked recoveries were between 94.2% and 103.8% and the extraction efficiencies were in the range of 95.5%-102.8%,which are better than those obtained by heat extraction.With the advantages of quickness,accuracy and good reproducibility,the method was suitable for the arsenic species detection and quality monitoring of animal derived traditional Chinese medicine and similar samples.