A new method was established to determine 10 kinds of fungicides（including sulfanilamide，benzimidazole，BIT，MBT，IPBC，PCMC，salicylanilide，OPP，TCMTB，OIT） in leather samples by ultrasonic extraction combined with ultrahigh performance liquid chromatography（UPLC）.The leather samples were ultrasonically extracted with methanol，and separated with T3 column，employing methanol and 0.1%（by volume） formic water as mobile phase by gradient elution．All of the samples were detected with diode-array detector.The 10 kinds of analytes presented favorable linearity in a certaim concentration range，with correlation coefficients（r2） not less than 0.999 3.The method quantitation limit（LOQ，S/N=10） of IPBC was 190.0 mg/kg，and the LOQs of other compounds were in the range of 4.0-8.0 mg/kg.The average recoveries（n=6） ranged from 81.3% to 104.9%，and the relative standard deviations （RSD，n=6） were in the range of 4.7%-11.2%.With the advantages of simple pre-treatment procedure and satisfactory separate effect，the developed method was suitable for the simultaneous determination of 10 kinds of fungicides in leather samples.