A method was established to determine the content of empagliflozin by 1H nuclear magnetic resonance spectroscopy（1H-NMR），using gemcitabine hydrochloride and zidovudine as the internal standard，repectively.NMR spectra were recorded using a standard 1D pulse sequence at a 30° flip angle and with DMSO-d6-D2O as the solvent.Thirty two scans of 32 K data points were acquired at a temperature of 303 K，a relaxation delay of 20 s and a line broad of 03 Hz.Under the optimal conditions，method validation indicated specificity，robustness and stability up to 24 h.A good linear relationship was obtained between peak area ratio and molar ratio of empagliflozin to the internal standard.For gemcitabine，the regression equation was y=0.009x-0.003（r2=0.999 9） with a molar ratio range of 0.512 5-1.953 8.For zidovudine，the regression equation was y=0.576x-0.026（r2=0.999 9） with a molar ratio range of 0.494 7-1.966 0.The contents of empagliflozin were 99.83%（n=3，RSD=0.06%）and 99.77%（n=3，RSD=0.19%）corresponding to the internal standards gemcitabine hydrochloride and zidovudine，respectively.The results were consistent with each other，indicating that the method had the advantages of accuracy，high sensitivity and good repeatability，and could be used for the quantification of new drugs.