The rapid quantitative detection of biochemical molecule has the advantages of simple handling，rapid detection and easy on-site analysis，thus it has a widespread application prospect in many fields such as medical diagnosis，food safety and environment monitoring.Molecular detection technology based on optical disc and optical drive can be used as the next generation molecular diagnostic tool.In this paper，a bran new quantitative detection of molecule based on bio-disc(BD) was proposed and studied around our previous research on BD detection based on CD/DVD technology.Compared with CD and DVD，Blu ray drive has a smaller focus laser size，which means higher detection throughput and sensitivity.The quantitation capability and the lateral resolution of this system were evaluated by preparing ink spot arrays with different sizes and darkness on the surface of BDs which were subsequently examined with a standard Blu-ray drive in conjunction with disc quality check software，and the Blu-ray detection system exhibits the higher sensitivity and lateral resolution（<100 μm）.In the end，biotin-streptavidin binding assay was successfully prepared on the surface of BDs，and the quantitative detection of streptavidin was achieved by a standard Blu-ray drive in conjunction with disc quality check software.