A method of oligosaccharide profiling test was developed based on high performance liquid chromatography-quadrurpole/ electrostatic field orbitrap high resolution mass spectrometry(HPLC-Q/Orbitrap MS).Through qualitative and quantitative analysis of maltotriose，maltotetraose，maltopentaose，maltohexaose and maltoheptaose in pure natural honey and starch syrup，trace of malt oligosaccharides that was not totally enzymatic hydrolyzed were found in starch syrup.Furthermore，the interference of maltoheptaose in all test samples was the smallest and had no impact on the quantitative analysis results.While，the qualitative or quantitative analysis of other oligosaccharides were interfered more or less.In the analysis of real samples，the starch syrup in honey adulteration could be judged by the retention time and relative abundance ratio of maltoheptaose，therefore the content of maltoheptaose in sample solution could be quantitatively determined through standard curve method.By adding standard test，the average recoveries of maltoheptaose at three spiked levels of 20，50 and 100 mg/kg were in the range of 75%-82% with relative standard deviations(RSD，n=5) of 3.1%-6.7%.The detection limit of this method was 0.1 μg/mL.The whole analysis time could be limited in 10 min.The method had the advantages of simple processing procedure，high accuracy，reliability，sensitivity，therefore it could be applied in the rapid detection of starch syrup in honey.