A poly amino acid film was modified on the surface of glassy carbon electrode by electro polymerization,then the copper metal organic frameworks(MOFs) with excellent electrical conductivity and catalytic ability were self-assembled on the surface of the modified electrode via carboxyl coordination.The voltammetric behaviors of metronidazole(MNZ) on the modified electrode and its electrocatalytic properties were studied.Thus,a MNZ electrochemical sensor with preeminent sensitivity was established.The method had a good linearity in the range of 1.0-100.0 μmol/L with a detection limit of 0.23 μmol/L,and was also successfully applied in the detecttion of MNZ in the honey sample.