A method for the determination of ten plant growth regulator residues in fruit and bean sprout by ultra performance liquid chromatography-electrospray tandem mass spectrometry(UPLC-MS/MS) was established. The samples were extracted by the stirring and ultrasound using formic acid-acetonitrile(1∶1000), and purified with the solid phase extraction column. The analytes were separated by using methanol-5mmol/L ammonium acetate as mobile phase. The identifications were achieved by the electrospray ionization in positive and negative mode(ESI+/ESI-) using the multiple reaction monitoring(MRM).The quantifications were performed by the matrix matched isotope-labelled internal standards. Under the optimal experimental conditions, the calibration curves of ten analytes showed good linearities in the concentration range of 0.1-50.0μg/L with correlation coefficients(r2) above 0.995. Recoveries were between 52.4% and 119.6% with RSDs less than 13.0%.The limits of detection were 0.2μg/kg. The method had the advantages of simple pretreatment, good purification and low matrix effects, and was suitable for the rapid, high-throughput quantitative analysis of plant growth regulator residues in fruit and bean sprout.