Chiral metal-organic frameworks(MOFs),as a novel type of porous material with many properties such as large surface area,diverse structure,adjustable pore size and good chemical stability,have aroused much attention in the field of chromatographic separation.In this paper,a homochiral MOF［Cu3(HL)2(L)2(bpy)3］·4H2O with 2D chiral network was synthesized through the reaction of two kinds of ligands((1R,2R)-1,2-cyclohexanedicarboxylic acid(H2L) and 4,4′-bipyridine(bpy)) with copper ion via a solvothermal approach.The homochiral MOF ［Cu3(HL)2(L)2(bpy)3］·4H2O was used as chiral stationary phase to fabricate a packed column for high performance liquid chromatography.To investigate the MOF′s chiral recognition ability,a series of racemates were separated on the homochiral MOF column under normal phase condition using n hexane-isopropanol as mobile phase.The homochiral MOF column exhibited a good resolving ability towards 10 kinds of chiral compounds,including alcohols,ketones,acids,epoxy compounds and ethers,etc.The reproducibility and stability of the homochiral MOF column were evaluated.The effect of the injected mass of chiral compound on separation was also investigated.Results indicated that the homochiral MOF column has a good reproducibility and stability for enantioseparation.