The dispersed multiwalled carbon nanotubes with carboxyl(f MWCNTs) were firstly dropped on the glass carbon electrode to allow a f-MWCNTs menbrance formed on the electrode surface,then chitosan(CS) was electrodeposited on the surface of the eletrode via potentiostatic method to develop a CS/f-MWCNTs/GCE which was used for the chiral recognition of tryptophan enantiomers.The surface morphology of the eletrode was characterized via scanning electron microscopy(SEM).Cyclic voltammetry(CV) and electrochemical impedance spectroscopy(EIS) were used to investigate the electrochemical behavior difference of the modified electrode.Differential pulse voltammetery(DPV) was employed to investigate the stereospecific recognition of tryptophan(Trp) enantiomers with a separation coefficient of 2.38.The results showed that the CS/f MWCNTs/GCE displayed a more strong DPV response for L-Trp than that for D-Trp in the linear range of 8.0×10-6-4.0×10-3 mol/L with a detection limit of 5.0×10-6 mol/L(S/N=3).Meanwhile,it provided a beneficial reference for the discrimination of other chiral enantiomers.