Organic nanomaterials（ONMs）generally refer to nanomaterials based on lipids,proteins,polysaccharides and polymers.In recent years,ONMs have been widely used in food,agriculture,and biomedical industries with the rapid development of nanotechnology.Although the unique properties of ONMs endow them great application potentials,they still have some potential toxicity to organisms.Some ONMs are difficult to degrade,and they might be discharged into the environment through human activities,and accumulated in the organisms.Therefore,the safety assessment on ONMs has attracted increasing research interest.This also raises new challenges toward their characterization and analytical methods.The analysis of ONMs often includes procedures of sample preparation,separation,characterization and detection.Many techniques are involved such as filtration,centrifugation,chromatography,electron microscopy,spectroscopy and mass spectrometry.The current progresses in the application and development of analytical methods of ONMs are summeried in this article.The perspectives on the future research in this area are also proposed.