The enzyme-free detection of glucose was achieved by using the nano-copper/graphene-chitosan composite matrix modified glass carbon electrode.The microstructure of the composite matrix was characterized by scanning electron microscopy(SEM),FT-IR spectrum and electrochemical impedance spectroscopy(EIS).The electrochemistry behavior of the modified electrode was investigated by cyclic voltammetry and chronoamperometry.The results showed that the modified electrode exhibited a good catalytic oxidation of glucose in 0.1 mol/L NaOH and with a wide linear range of 5.6×10-5-1.2×10-3 mol/L and a low detection limit of 2.3×10-5 mol/L(at signal/noise ratio S/N = 3).The modified electrode could be used to detect the real samples with a good stability and repeatability.