In this study,the ultra high performance liquid chromatography-Q exactive hybrid quadrupole-orbitrap high-resolution accurate mass spectrometry(UHPLC-Q-Orbitrap-HRMS) was firstly applied in the analysis of the plasma from the glioma patients and the normal control group to find the differential metabolites,which could provide the scientific evidence for clinical diagnosis and pathogenesis research.The spectra obtained by UHPLC-Q-Orbitrap HRMS were conducted to peak identification,peak matching and denoising.The data of metabolomics were statistically analyzed by using principal component analysis and orthogonal partial least-squares-discriminant analysis method to screen the differential metabolites with VIP>10 and P<005,which were investigated to further evaluate the diagnostic ability.The results showed that the plasma metabolic profiles of glioma patients were dramatically altered,and ten potential biomarkers were identified.The metabolites of leucine,valine,tryptophan,choline and taurine were significantly reduced in glioma patients,while histidine,citric acid,lactic acid,creatine and pyruvic acid were obviously increased compared with the normal controls(P<005),which suggested that amino acid and energy metabolism might play an important role in the development of glioma.In addition,the different metabolites showed good diagnostic ability toward gliomas(AUC>08),which could be used as potential diagnostic biomarkers.