An analytical method was successfully developed for the rapid detection of pyrethrins in vegetables using ultra high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS).Vegetable samples were extracted with acetonitrile.After salting out,the solvent extracts were determined by UHPLC-MS/MS without further cleanup steps.The positive ion mode and multiple reaction monitoring(MRM) mode were used to identify and quantify pyrethrins in vegetables.Results showed that all six compounds had good linearity in their respective concentration ranges with the correlation coefficients of 0.999 5-0.999 9.Their average recoveries at spiked levels of 0.05,0.1, 0.5 mg/kg ranged from 73.9%to 109.3%with relative standard deviations(RSDs) of 0.4%-7.7%.The limits of quantitation(LOQs) were 0.01 mg/kg for pyrethrinⅠand pyrethrinⅡ,and 0.05 mg/kg for cinerinⅠ,cinerinⅡ,jasmolinⅠand jasmolinⅡ.Both LOQs referred to total pyrethrins.Results showed that this method was simple,rapid and sensitive,and was suitable for the rapid determination of pyrethrins in vegetables.
关键词
农药残留蔬菜除虫菊素超高效液相色谱-串联质谱
Keywords
pesticide residuesvegetablepyrethrinsultra high performance liquid chromatography-tandem mass spectrometry