A high performance liquid chromatographic(HPLC) method with microwave hydrolysis derivation was developed for the simultaneous determination of 17 amino acids in feed.The method was based on pre-column derivatization with 2,4-dinitro-chlorobenzene.The compounds were separated on a C18 column.And the conditions of microwave hydrolysis were optimized.The detection was performed using a diode array detector at 360 nm.The calibration curves of 17 amino acids all exhibited good linearities in the range of 2.5-50 mg/L.The correlation coefficients(r2) for the determination varied between 0.990 7 and 0.999 9 with the relative standard deviations(RSDs) of 0.88% to 4.2%,and the recoveries of 17 amino acids were in the range of 90.6%-107.2% with the limits of detection of 0.15-2.37 mg/L.The results showed that the results of microwave hydrolysis in 16 min at 150 ℃ are basically the same as those of traditional heating hydrolysis(110 ℃,24 h).With the advantages of short analysis time,high sensitivity,the method could be used for the determination of amino acids in feed.