An analytical method was established for the determination of 15 polycyclic aromatic hydrocarbons(PAHs) in infant formula and rice by high performance liquid chromatography with fluorescence detector(HPLC-FLD).The samples were extracted with acetonitrile,purified by PSA dispersed solid phase extraction,separated on a Waters PAH special column with acetonitrile-water as mobile phase by gradient elution,and detected with the fluorescence detector.These compounds were quantified by the external standard method.Under the optimal conditions,15 PAHs were effectively separated.The linear ranges were 1.0-50 μg/L with correlation coefficients above 0.995.The limits of detection(S/N=3) for 15 PAHs were between 0.05 μg/kg and 0.3 μg/kg.The recoveries were in the range of 86.5% -106.8% with relative standard deviations(n=6) of 0.7%-7.5%.The method was simple and highly sensitive,and was suitable for the determination of polycyclic aromatic hydrocarbons in infant formula and rice.