A method of solid-phase extraction(SPE) and high performance liquid chromatography-tandem mass spectrometry(SPE/HPLC-MS/MS) was developed for the simultaneous determination of 24 pesticides in water.The samples were extracted with acetonitrile,then enriched and purified with solid-phase extraction cartridges.Acetonitrile and 0.1%(by volume) formic acid aqueous solution were used as mobile phases.Target pesticides were analyzed by HPLC-MS/MS in positive electrospray ionization(ESI+) under multiple reaction monitoring(MRM) mode.Results showed that 24 pesticides were separated in 25 min with excellent linear relationships(r2≥0.998).The recoveries at spiked levels of 5,20,100 μg/L in water samples ranged from 65.9%-127.8% with relative standard deviations(RSDs) of 0.7%-14.2%.The limits of detection were in the range of 0.05-0.71 ng/L.This method was applied in the detection of the water samples from 10 estuaries and 2 reservoirs in Dalian，and 10 pesticides were detected in the concentration range of 0.2-558.3 ng/L.The proposed SPE/HPLC-MS/MS method is efficient and sensitive,and is suitable for the determination of various pesticides in actual water samples.