A rapid method for the identification of constituents in Yangxinshi tablets was established by high performance liquid chromatography-time of flight mass spectrometry(HPLC-TOF/MS).Simultaneously,the quantitation on the sample was performed by high performance liquid chromatography-triple quadrupole mass spectrometry(HPLC-MS/MS).30 kinds of constituents in Yangxinshi tablets were identified by HPLC-TOF/MS in the running conditions as follows:chromatographic column:an Agilent ZORBAX Eclipse Plus C18 column(100 mm×30 mm,18 μm),mobile phase:acetonitrile-01% formic acid solution,elution type:gradient elution,flow rate:02 mL·min-1,column temperature:30 ℃,MS scanning mode:electrospray in both positive and negative ion.The HPLC-MS/MS method was operated under the following conditions:chromatographic column:a Waters XBridge BEH C18 column(150 mm×46 mm,25 μm),mobile phase:acetonitrile-01% formic acid solution,elution type:gradient elution,flow rate:04 mL·min-1,column temperature:30 ℃,MS scanning mode:multiple reaction monitoring(MRM) mode with electrospray ion source in both positive and negative ion.16 main constituents in Yangxinshi tablets were determined by HPLC-MS/MS.There were excellent linear responses for the peak areas of the analytes to their concentrations with the correlation coefficients(r) not less than 0997.The limits of detection(LODs) and quantitation(LOQs) for 16 constituents were in the range of 0039 1-781 μg/L and 0156-312 μg/L,respectively.The average recoveries were in the range of 968%-1038% with the relative standard deviations of 10%-35%.With the advantages of simple operation,accuracy and acceptable reproducibility,the developed method could be applied in the qualitation and quantitation of constituents in Yangxinshi tablets.Therefore,it could provide a reference for the quality control,further study and application of Yangxinshi tablets.